Non-Antibiotic Immunomodulatory Combination Treatment for <i>Staphylococcus aureus</i> Infections: <i>In vitro</i> Preliminary Studies I

Despite advancements in prevention measures, infectious mastitis is a common disease of economic significance cattle. Clinical affects 20 to 25% cows each year. Staphylococcus aureus (S. aureus) causes an estimated 10-11.7% clinical bovine cases the US. Antibiotic therapy tends fail 50% time and nature vaccine immunity usually fails, due S. aureus’ evasion mechanisms, leading significant morbidity, chronic mastitis, culling such cows. Specific IgYs have been found inhibit growth vitro, but produced especially for experiments, commercially available presentations not compared IgY by naturally exposed chickens. In present study, we compare impact two sources IgY. was obtained purified from eggs chickens (RIR IgY). Their effect on vitro commercial chicken anti-SpA (IgY). This done mixing tryptic soy broth cultures with 5, 25, or 125 µg/mL RIR (3 tubes per concentration). The result evaluated plate count method. Next, treated control broths were sampled plated at 4, 8, 12 h. (commercial) showed powerful inhibitory over aureus. sustained h when respectively control. Though significant; however, it subsided after 8 Based our findings, may effective component against which more observed than are